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Comparison of immunophenotypes between CAR.MUC1-41BB ζ and CAR.MUC1-CD28 ζ . The phenotypic analyses of CAR T cells were performed on days 4, 11, and 14 after transduction. (a) Representative flow cytometry plot demonstrating the cell subset analyzed with fluorochrome-conjugated anti-CD3 and anti-CD56 on day 4 after T cell transduction. (b) Bar graph represents the percentage of CD3 + T cells (mean ± S.E.M from 8 donors). (c) Representative flow cytometry plot at 4 and 11 after transduction. (d) Mean percentage of CD8 + : CD4 + ratio on day 4 and day 11 after transduction is shown. Data shown are mean ± S.E.M from 6 donors. (e) Representative flow cytometry analysis of memory phenotype analyzed on days 4, 11, and 14 after transduction; CAR T cells were expanded in culture media containing IL-2 50 U/ml and stained with <t>anti-CD45RO</t> and anti-CD62L antibody (TEM: CD45RO + CD62L − , TCM: CD45RO + CD62L + , TN: CD45RO − CD62L + , and TE: CD45RO − CD62L − ). (f) The mean percentage of naïve (TN), central memory (TCM), effector memory (TEM), and terminal effector (TE) T cells. Data represents as mean ± S.E.M ( n = 6).
Cd45ro Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Comparison of immunophenotypes between CAR.MUC1-41BB ζ and CAR.MUC1-CD28 ζ . The phenotypic analyses of CAR T cells were performed on days 4, 11, and 14 after transduction. (a) Representative flow cytometry plot demonstrating the cell subset analyzed with fluorochrome-conjugated anti-CD3 and anti-CD56 on day 4 after T cell transduction. (b) Bar graph represents the percentage of CD3 + T cells (mean ± S.E.M from 8 donors). (c) Representative flow cytometry plot at 4 and 11 after transduction. (d) Mean percentage of CD8 + : CD4 + ratio on day 4 and day 11 after transduction is shown. Data shown are mean ± S.E.M from 6 donors. (e) Representative flow cytometry analysis of memory phenotype analyzed on days 4, 11, and 14 after transduction; CAR T cells were expanded in culture media containing IL-2 50 U/ml and stained with <t>anti-CD45RO</t> and anti-CD62L antibody (TEM: CD45RO + CD62L − , TCM: CD45RO + CD62L + , TN: CD45RO − CD62L + , and TE: CD45RO − CD62L − ). (f) The mean percentage of naïve (TN), central memory (TCM), effector memory (TEM), and terminal effector (TE) T cells. Data represents as mean ± S.E.M ( n = 6).
Mast Cell Tryptase Antibody, Anti Human, Apc, Readye Lease, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Comparison of immunophenotypes between CAR.MUC1-41BB ζ and CAR.MUC1-CD28 ζ . The phenotypic analyses of CAR T cells were performed on days 4, 11, and 14 after transduction. (a) Representative flow cytometry plot demonstrating the cell subset analyzed with fluorochrome-conjugated anti-CD3 and anti-CD56 on day 4 after T cell transduction. (b) Bar graph represents the percentage of CD3 + T cells (mean ± S.E.M from 8 donors). (c) Representative flow cytometry plot at 4 and 11 after transduction. (d) Mean percentage of CD8 + : CD4 + ratio on day 4 and day 11 after transduction is shown. Data shown are mean ± S.E.M from 6 donors. (e) Representative flow cytometry analysis of memory phenotype analyzed on days 4, 11, and 14 after transduction; CAR T cells were expanded in culture media containing IL-2 50 U/ml and stained with <t>anti-CD45RO</t> and anti-CD62L antibody (TEM: CD45RO + CD62L − , TCM: CD45RO + CD62L + , TN: CD45RO − CD62L + , and TE: CD45RO − CD62L − ). (f) The mean percentage of naïve (TN), central memory (TCM), effector memory (TEM), and terminal effector (TE) T cells. Data represents as mean ± S.E.M ( n = 6).
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Comparison of immunophenotypes between CAR.MUC1-41BB ζ and CAR.MUC1-CD28 ζ . The phenotypic analyses of CAR T cells were performed on days 4, 11, and 14 after transduction. (a) Representative flow cytometry plot demonstrating the cell subset analyzed with fluorochrome-conjugated anti-CD3 and anti-CD56 on day 4 after T cell transduction. (b) Bar graph represents the percentage of CD3 + T cells (mean ± S.E.M from 8 donors). (c) Representative flow cytometry plot at 4 and 11 after transduction. (d) Mean percentage of CD8 + : CD4 + ratio on day 4 and day 11 after transduction is shown. Data shown are mean ± S.E.M from 6 donors. (e) Representative flow cytometry analysis of memory phenotype analyzed on days 4, 11, and 14 after transduction; CAR T cells were expanded in culture media containing IL-2 50 U/ml and stained with <t>anti-CD45RO</t> and anti-CD62L antibody (TEM: CD45RO + CD62L − , TCM: CD45RO + CD62L + , TN: CD45RO − CD62L + , and TE: CD45RO − CD62L − ). (f) The mean percentage of naïve (TN), central memory (TCM), effector memory (TEM), and terminal effector (TE) T cells. Data represents as mean ± S.E.M ( n = 6).
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Comparison of immunophenotypes between CAR.MUC1-41BB ζ and CAR.MUC1-CD28 ζ . The phenotypic analyses of CAR T cells were performed on days 4, 11, and 14 after transduction. (a) Representative flow cytometry plot demonstrating the cell subset analyzed with fluorochrome-conjugated anti-CD3 and anti-CD56 on day 4 after T cell transduction. (b) Bar graph represents the percentage of CD3 + T cells (mean ± S.E.M from 8 donors). (c) Representative flow cytometry plot at 4 and 11 after transduction. (d) Mean percentage of CD8 + : CD4 + ratio on day 4 and day 11 after transduction is shown. Data shown are mean ± S.E.M from 6 donors. (e) Representative flow cytometry analysis of memory phenotype analyzed on days 4, 11, and 14 after transduction; CAR T cells were expanded in culture media containing IL-2 50 U/ml and stained with <t>anti-CD45RO</t> and anti-CD62L antibody (TEM: CD45RO + CD62L − , TCM: CD45RO + CD62L + , TN: CD45RO − CD62L + , and TE: CD45RO − CD62L − ). (f) The mean percentage of naïve (TN), central memory (TCM), effector memory (TEM), and terminal effector (TE) T cells. Data represents as mean ± S.E.M ( n = 6).
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Image Search Results


Comparison of immunophenotypes between CAR.MUC1-41BB ζ and CAR.MUC1-CD28 ζ . The phenotypic analyses of CAR T cells were performed on days 4, 11, and 14 after transduction. (a) Representative flow cytometry plot demonstrating the cell subset analyzed with fluorochrome-conjugated anti-CD3 and anti-CD56 on day 4 after T cell transduction. (b) Bar graph represents the percentage of CD3 + T cells (mean ± S.E.M from 8 donors). (c) Representative flow cytometry plot at 4 and 11 after transduction. (d) Mean percentage of CD8 + : CD4 + ratio on day 4 and day 11 after transduction is shown. Data shown are mean ± S.E.M from 6 donors. (e) Representative flow cytometry analysis of memory phenotype analyzed on days 4, 11, and 14 after transduction; CAR T cells were expanded in culture media containing IL-2 50 U/ml and stained with anti-CD45RO and anti-CD62L antibody (TEM: CD45RO + CD62L − , TCM: CD45RO + CD62L + , TN: CD45RO − CD62L + , and TE: CD45RO − CD62L − ). (f) The mean percentage of naïve (TN), central memory (TCM), effector memory (TEM), and terminal effector (TE) T cells. Data represents as mean ± S.E.M ( n = 6).

Journal: Journal of Immunology Research

Article Title: An In Vitro Comparison of Costimulatory Domains in Chimeric Antigen Receptor T Cell for Breast Cancer Treatment

doi: 10.1155/2022/2449373

Figure Lengend Snippet: Comparison of immunophenotypes between CAR.MUC1-41BB ζ and CAR.MUC1-CD28 ζ . The phenotypic analyses of CAR T cells were performed on days 4, 11, and 14 after transduction. (a) Representative flow cytometry plot demonstrating the cell subset analyzed with fluorochrome-conjugated anti-CD3 and anti-CD56 on day 4 after T cell transduction. (b) Bar graph represents the percentage of CD3 + T cells (mean ± S.E.M from 8 donors). (c) Representative flow cytometry plot at 4 and 11 after transduction. (d) Mean percentage of CD8 + : CD4 + ratio on day 4 and day 11 after transduction is shown. Data shown are mean ± S.E.M from 6 donors. (e) Representative flow cytometry analysis of memory phenotype analyzed on days 4, 11, and 14 after transduction; CAR T cells were expanded in culture media containing IL-2 50 U/ml and stained with anti-CD45RO and anti-CD62L antibody (TEM: CD45RO + CD62L − , TCM: CD45RO + CD62L + , TN: CD45RO − CD62L + , and TE: CD45RO − CD62L − ). (f) The mean percentage of naïve (TN), central memory (TCM), effector memory (TEM), and terminal effector (TE) T cells. Data represents as mean ± S.E.M ( n = 6).

Article Snippet: For cell surface marker analysis, the following antibodies were used: CD56-PE (HCD56/Cat#130-114-551), CD4-FITC (OKT4/130-114-531), CD8-APC (SK1/130-110-679), CD45RO-APC (UCHL1/130-113-556), CD62L-FITC (DREG-56/130-112-077), CD25-APC (BC96/130-113-284) (Miltenyi Biotec, Bergisch Gladbach, Germany), PD1-FITC (EH12.2H7/329904), LAG-3-FITC (11C3C65/369308), TIM-3-APC (F38-2E2/345012), and CD3-PerCP (clone OKT3/317336) (BioLegend, San Diego, CA, USA).

Techniques: Comparison, Transduction, Flow Cytometry, Staining